Chromatin Immunoprecipitation:Article Title:
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used AP2-alpha (Santa Cruz Biotechnology; sc-12726; clone 3B5; 1:200); AP2-gamma (R&D Systems; AF5059; 1:500); AP2-gamma (Santa Cruz Biotechnology, sc-12762; clone 6E4/4; 1:200); Brachyury (R&D Systems; AF2085; 1:500); CDX2 (BioGenex; MU392-UC; clone 4 nature portfolio | reporting sum m ary M arch 2021 CDX2-88; 1:200); CER1 (R&D Systems; AF1075; 1:500); Cytokeratin 7 (Aligent; M7018; clone OV-TL 12/30; 1:100); E-Cadherin (BD Biosciences; 610182; clone 36; 1:200); EOMES (Abcam; ab23345; 1:200); FOXA2 (R&D Systems; AF2400; 1:200); GATA2 (Novus Biologics; NBMP1-82581; 1:200); GATA3 (Abcam; ab199428; clone EPR16651; 1:500); GATA4 (Santa Cruz Biotechnology; sc-25310; clone G-4; 1:200); GATA4 (ThermoFisher Scientific; 14-9980-82; clone eBioEvan; 1:500); GATA6 (R&D Systems; AF1700; 1:500); GATA6 (Cell Signaling Technology; 5851; clone D61E4; 1:2000); GFP (Abcam; ab13970; 1:1000); GFP (Nacalai USA; GF090R; clone GF090R; 1:1000); HAND1 (DSHB; PCRP-HAND1-2A9; clone 2A9 1:200); HNF4-alpha (Abcam; ab201460; clone EPR16885-99; 1:2000); ISL1 (DSHB; PCRP-ISL1-1A9; clone 1A9; 1:100); Laminin (Sigma Aldrich; L9393; 1:200); N-Cadherin (Abcam; ab98952; clone 5D5; 1:200); NANOG (Cell Signaling Technology; 4903; clone D73G4; 1:200); OCT3/4 (Santa Cruz Biotechnology; sc-5279; clone C-10; 1:100); OTX2 (R&D Systems; AF1979; 1:1000); phospho-SMAD1/5 (Cell Signaling Technology; 9516S; clone 41D10; 1:200); Smad2/3 (Cell Signaling Technology; 8685S; clone D7G7; 1:200); SOX17 (R&D Systems; AF1924; 1:500); SOX2 (ThermoFisher Scientific; 14-9811-82; clone Btjce; 1:500); TBX20 (R&D Systems; MAB8124; clone 668710; 1:100); VTCN1 (Abcam; ab209242; clone EPR20236; 1:200). .. AlexaFluor-405 Donkey Anti-Mouse (ThermoFisher Scientific; A48257; 1:500); AlexaFluor-488 Donkey Anti-Rat (ThermoFisher Scientific; A-21208; 1:500); AlexFluor-488 Donkey Anti-Mouse (ThermoFisher Scientific; A-21202; 1:500); AlexaFluor-488 Donkey Anti-Goat (ThermoFisher Scientific; A-11055; 1:500); AlexaFluor-568 Donkey Anti-Rabbit (ThermoFisher Scientific; A10042; 1:500); AlexaFluor-568 Donkey Anti-Rat (ThermoFisher Scientific; A78946; 1:500); AlexaFluor-568 Donkey Anti-Mouse (ThermoFisher Scientific; A10037; 1:500); AlexaFluor-647 Donkey Anti-Rat (ThermoFisher Scientific; A78947; 1:500); AlexaFluor-647 Donkey AntiGoat (ThermoFisher Scientific; A-21447; 1:500); AlexaFluor-647 Donkey Anti-Rabbit (Thermofisher Scientific; A-31573; 1:500); AlexaFluor-647 Donkey Anti-Mouse (ThermoFisher Scientific; A32787; 1:500).
Article Title: Elucidating causative gene variants in hereditary Parkinson's disease in the Global Parkinson's Genetics Program (GP2).
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used AP2-alpha (Santa Cruz Biotechnology; sc-12726; clone 3B5; 1:200); AP2-gamma (R&D Systems; AF5059; 1:500); AP2-gamma (Santa Cruz Biotechnology, sc-12762; clone 6E4/4; 1:200); Brachyury (R&D Systems; AF2085; 1:500); CDX2 (BioGenex; MU392-UC; clone 4 nature portfolio | reporting sum m ary M arch 2021 CDX2-88; 1:200); CER1 (R&D Systems; AF1075; 1:500); Cytokeratin 7 (Aligent; M7018; clone OV-TL 12/30; 1:100); E-Cadherin (BD Biosciences; 610182; clone 36; 1:200); EOMES (Abcam; ab23345; 1:200); FOXA2 (R&D Systems; AF2400; 1:200); GATA2 (Novus Biologics; NBMP1-82581; 1:200); GATA3 (Abcam; ab199428; clone EPR16651; 1:500); GATA4 (Santa Cruz Biotechnology; sc-25310; clone G-4; 1:200); GATA4 (ThermoFisher Scientific; 14-9980-82; clone eBioEvan; 1:500); GATA6 (R&D Systems; AF1700; 1:500); GATA6 (Cell Signaling Technology; 5851; clone D61E4; 1:2000); GFP (Abcam; ab13970; 1:1000); GFP (Nacalai USA; GF090R; clone GF090R; 1:1000); HAND1 (DSHB; PCRP-HAND1-2A9; clone 2A9 1:200); HNF4-alpha (Abcam; ab201460; clone EPR16885-99; 1:2000); ISL1 (DSHB; PCRP-ISL1-1A9; clone 1A9; 1:100); Laminin (Sigma Aldrich; L9393; 1:200); N-Cadherin (Abcam; ab98952; clone 5D5; 1:200); NANOG (Cell Signaling Technology; 4903; clone D73G4; 1:200); OCT3/4 (Santa Cruz Biotechnology; sc-5279; clone C-10; 1:100); OTX2 (R&D Systems; AF1979; 1:1000); phospho-SMAD1/5 (Cell Signaling Technology; 9516S; clone 41D10; 1:200); Smad2/3 (Cell Signaling Technology; 8685S; clone D7G7; 1:200); SOX17 (R&D Systems; AF1924; 1:500); SOX2 (ThermoFisher Scientific; 14-9811-82; clone Btjce; 1:500); TBX20 (R&D Systems; MAB8124; clone 668710; 1:100); VTCN1 (Abcam; ab209242; clone EPR20236; 1:200). .. AlexaFluor-405 Donkey Anti-Mouse (ThermoFisher Scientific; A48257; 1:500); AlexaFluor-488 Donkey Anti-Rat (ThermoFisher Scientific; A-21208; 1:500); AlexFluor-488 Donkey Anti-Mouse (ThermoFisher Scientific; A-21202; 1:500); AlexaFluor-488 Donkey Anti-Goat (ThermoFisher Scientific; A-11055; 1:500); AlexaFluor-568 Donkey Anti-Rabbit (ThermoFisher Scientific; A10042; 1:500); AlexaFluor-568 Donkey Anti-Rat (ThermoFisher Scientific; A78946; 1:500); AlexaFluor-568 Donkey Anti-Mouse (ThermoFisher Scientific; A10037; 1:500); AlexaFluor-647 Donkey Anti-Rat (ThermoFisher Scientific; A78947; 1:500); AlexaFluor-647 Donkey AntiGoat (ThermoFisher Scientific; A-21447; 1:500); AlexaFluor-647 Donkey Anti-Rabbit (Thermofisher Scientific; A-31573; 1:500); AlexaFluor-647 Donkey Anti-Mouse (ThermoFisher Scientific; A32787; 1:500).
Article Title: Global reorganization of genome architecture at the transition to gametogenesis.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used H3K9me3 (Diagenode, C15410056, 1:400,IF) H3K9me3 (Diagenode, C15410193, 1:400,IF) H4K20me3 (Abcam, ab78517, 1:200, IF) H3K9me2 (Diagenode, C15410060, 1:200, IF) Pan-H3 (Abcam, Ab1791, 1:10000, WB) CENPA ((IF preferred), Cell signaling 2048,1:200, IF) CENPA (Cell Signaling, 2186T, for Human, 1:200, IF) CYCLIN B1 (Cell Signaling, 4138s, 1:200, IF) RAD21 (Abcam, 154769, 1:200, IF) CTCF (Cell Signaling, 2899, 1:200, IF) ATRX (Santa Cruz, SC15408, 1:200, IF) HP1alpha (Cell Signaling, 2616S, 1:200, IF) PCNA (Cell Signaling, 2586, 1:200, IF) MVH (Abcam, AB13840, 1:400, IF) MVH (Abcam, AB27591, 1:400, IF) DAZL (Abcam, 34139, 1:400, IF)) OCT4 (BD, 611202, 1:200, IF) OCT4 (Abcam, 181557, 1:200, IF) SCP3 (Santa Cruz, SC-74569, 1:200, IF) LAMINA/C (Cell Signaling, 4777, 1:200, IF) LAMIN B1 (Santa Cruz, SC6216, 1:200, IF) LAMIN B1 (Santa Cruz, SC374015), 1:200, IF LBR (Abcam, Ab232731, 1:200, IF) Ki67 (Cell Signaling, 99129, 1:200, IF) ANTI-GFP (Abcam, Ab5450, 1:200, IF) AP2 gamma (Santa Cruz, SC-12762, 1:200, IF) ANTI-RFP (Abcam, 62341, 1:200, IF) Anti-human/mouse SSEA1 Alexa Fluor647 (ebioscience, 518813, 1:20, FACS) Goat anti mouse Alexafluor 488 (Thermo Fisher, A11029, 1:500, IF) Goat anti mouse Alexafluor 568 (Thermo Fisher, A21144, 1:500, IF) Goat anti mouse Alexafluor 647 (Thermo Fisher, A-21235, 1:500, IF) Goat anti rabbit Alexafluor 488 (Thermo Fisher, A11034, 1:500, IF) Goat anti Rabbit Alexafluor 568 (Thermo Fisher, A11036, 1:500, IF) Goat anti Rabbit Alexafluor 647 (Thermo Fisher, A21245, 1:500, IF) Donkey anti-Mouse IgG Secondary Antibody, HRP (Thermo Fisher, A16011, 1:10000, WB) Goat anti-Rabbit IgG Secondary Antibody, HRP (Thermo Fisher, A16096, 1:10000, WB)) Validation All antibodies were purchased from the commercial vendors and were validated by their vendors. .. AES0146 : Dnmt1-/-Dnmt3a-/-Dnmt3b-/- ES (clone19), (PMID: 16824199) J1 ES cells were purchased from ATCC(SCRC-1010).
Flow Cytometry:Article Title:
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used AP2-alpha (Santa Cruz Biotechnology; sc-12726; clone 3B5; 1:200); AP2-gamma (R&D Systems; AF5059; 1:500); AP2-gamma (Santa Cruz Biotechnology, sc-12762; clone 6E4/4; 1:200); Brachyury (R&D Systems; AF2085; 1:500); CDX2 (BioGenex; MU392-UC; clone 4 nature portfolio | reporting sum m ary M arch 2021 CDX2-88; 1:200); CER1 (R&D Systems; AF1075; 1:500); Cytokeratin 7 (Aligent; M7018; clone OV-TL 12/30; 1:100); E-Cadherin (BD Biosciences; 610182; clone 36; 1:200); EOMES (Abcam; ab23345; 1:200); FOXA2 (R&D Systems; AF2400; 1:200); GATA2 (Novus Biologics; NBMP1-82581; 1:200); GATA3 (Abcam; ab199428; clone EPR16651; 1:500); GATA4 (Santa Cruz Biotechnology; sc-25310; clone G-4; 1:200); GATA4 (ThermoFisher Scientific; 14-9980-82; clone eBioEvan; 1:500); GATA6 (R&D Systems; AF1700; 1:500); GATA6 (Cell Signaling Technology; 5851; clone D61E4; 1:2000); GFP (Abcam; ab13970; 1:1000); GFP (Nacalai USA; GF090R; clone GF090R; 1:1000); HAND1 (DSHB; PCRP-HAND1-2A9; clone 2A9 1:200); HNF4-alpha (Abcam; ab201460; clone EPR16885-99; 1:2000); ISL1 (DSHB; PCRP-ISL1-1A9; clone 1A9; 1:100); Laminin (Sigma Aldrich; L9393; 1:200); N-Cadherin (Abcam; ab98952; clone 5D5; 1:200); NANOG (Cell Signaling Technology; 4903; clone D73G4; 1:200); OCT3/4 (Santa Cruz Biotechnology; sc-5279; clone C-10; 1:100); OTX2 (R&D Systems; AF1979; 1:1000); phospho-SMAD1/5 (Cell Signaling Technology; 9516S; clone 41D10; 1:200); Smad2/3 (Cell Signaling Technology; 8685S; clone D7G7; 1:200); SOX17 (R&D Systems; AF1924; 1:500); SOX2 (ThermoFisher Scientific; 14-9811-82; clone Btjce; 1:500); TBX20 (R&D Systems; MAB8124; clone 668710; 1:100); VTCN1 (Abcam; ab209242; clone EPR20236; 1:200). .. AlexaFluor-405 Donkey Anti-Mouse (ThermoFisher Scientific; A48257; 1:500); AlexaFluor-488 Donkey Anti-Rat (ThermoFisher Scientific; A-21208; 1:500); AlexFluor-488 Donkey Anti-Mouse (ThermoFisher Scientific; A-21202; 1:500); AlexaFluor-488 Donkey Anti-Goat (ThermoFisher Scientific; A-11055; 1:500); AlexaFluor-568 Donkey Anti-Rabbit (ThermoFisher Scientific; A10042; 1:500); AlexaFluor-568 Donkey Anti-Rat (ThermoFisher Scientific; A78946; 1:500); AlexaFluor-568 Donkey Anti-Mouse (ThermoFisher Scientific; A10037; 1:500); AlexaFluor-647 Donkey Anti-Rat (ThermoFisher Scientific; A78947; 1:500); AlexaFluor-647 Donkey AntiGoat (ThermoFisher Scientific; A-21447; 1:500); AlexaFluor-647 Donkey Anti-Rabbit (Thermofisher Scientific; A-31573; 1:500); AlexaFluor-647 Donkey Anti-Mouse (ThermoFisher Scientific; A32787; 1:500).
Article Title: Elucidating causative gene variants in hereditary Parkinson's disease in the Global Parkinson's Genetics Program (GP2).
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used AP2-alpha (Santa Cruz Biotechnology; sc-12726; clone 3B5; 1:200); AP2-gamma (R&D Systems; AF5059; 1:500); AP2-gamma (Santa Cruz Biotechnology, sc-12762; clone 6E4/4; 1:200); Brachyury (R&D Systems; AF2085; 1:500); CDX2 (BioGenex; MU392-UC; clone 4 nature portfolio | reporting sum m ary M arch 2021 CDX2-88; 1:200); CER1 (R&D Systems; AF1075; 1:500); Cytokeratin 7 (Aligent; M7018; clone OV-TL 12/30; 1:100); E-Cadherin (BD Biosciences; 610182; clone 36; 1:200); EOMES (Abcam; ab23345; 1:200); FOXA2 (R&D Systems; AF2400; 1:200); GATA2 (Novus Biologics; NBMP1-82581; 1:200); GATA3 (Abcam; ab199428; clone EPR16651; 1:500); GATA4 (Santa Cruz Biotechnology; sc-25310; clone G-4; 1:200); GATA4 (ThermoFisher Scientific; 14-9980-82; clone eBioEvan; 1:500); GATA6 (R&D Systems; AF1700; 1:500); GATA6 (Cell Signaling Technology; 5851; clone D61E4; 1:2000); GFP (Abcam; ab13970; 1:1000); GFP (Nacalai USA; GF090R; clone GF090R; 1:1000); HAND1 (DSHB; PCRP-HAND1-2A9; clone 2A9 1:200); HNF4-alpha (Abcam; ab201460; clone EPR16885-99; 1:2000); ISL1 (DSHB; PCRP-ISL1-1A9; clone 1A9; 1:100); Laminin (Sigma Aldrich; L9393; 1:200); N-Cadherin (Abcam; ab98952; clone 5D5; 1:200); NANOG (Cell Signaling Technology; 4903; clone D73G4; 1:200); OCT3/4 (Santa Cruz Biotechnology; sc-5279; clone C-10; 1:100); OTX2 (R&D Systems; AF1979; 1:1000); phospho-SMAD1/5 (Cell Signaling Technology; 9516S; clone 41D10; 1:200); Smad2/3 (Cell Signaling Technology; 8685S; clone D7G7; 1:200); SOX17 (R&D Systems; AF1924; 1:500); SOX2 (ThermoFisher Scientific; 14-9811-82; clone Btjce; 1:500); TBX20 (R&D Systems; MAB8124; clone 668710; 1:100); VTCN1 (Abcam; ab209242; clone EPR20236; 1:200). .. AlexaFluor-405 Donkey Anti-Mouse (ThermoFisher Scientific; A48257; 1:500); AlexaFluor-488 Donkey Anti-Rat (ThermoFisher Scientific; A-21208; 1:500); AlexFluor-488 Donkey Anti-Mouse (ThermoFisher Scientific; A-21202; 1:500); AlexaFluor-488 Donkey Anti-Goat (ThermoFisher Scientific; A-11055; 1:500); AlexaFluor-568 Donkey Anti-Rabbit (ThermoFisher Scientific; A10042; 1:500); AlexaFluor-568 Donkey Anti-Rat (ThermoFisher Scientific; A78946; 1:500); AlexaFluor-568 Donkey Anti-Mouse (ThermoFisher Scientific; A10037; 1:500); AlexaFluor-647 Donkey Anti-Rat (ThermoFisher Scientific; A78947; 1:500); AlexaFluor-647 Donkey AntiGoat (ThermoFisher Scientific; A-21447; 1:500); AlexaFluor-647 Donkey Anti-Rabbit (Thermofisher Scientific; A-31573; 1:500); AlexaFluor-647 Donkey Anti-Mouse (ThermoFisher Scientific; A32787; 1:500).
Article Title: Global reorganization of genome architecture at the transition to gametogenesis.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used H3K9me3 (Diagenode, C15410056, 1:400,IF) H3K9me3 (Diagenode, C15410193, 1:400,IF) H4K20me3 (Abcam, ab78517, 1:200, IF) H3K9me2 (Diagenode, C15410060, 1:200, IF) Pan-H3 (Abcam, Ab1791, 1:10000, WB) CENPA ((IF preferred), Cell signaling 2048,1:200, IF) CENPA (Cell Signaling, 2186T, for Human, 1:200, IF) CYCLIN B1 (Cell Signaling, 4138s, 1:200, IF) RAD21 (Abcam, 154769, 1:200, IF) CTCF (Cell Signaling, 2899, 1:200, IF) ATRX (Santa Cruz, SC15408, 1:200, IF) HP1alpha (Cell Signaling, 2616S, 1:200, IF) PCNA (Cell Signaling, 2586, 1:200, IF) MVH (Abcam, AB13840, 1:400, IF) MVH (Abcam, AB27591, 1:400, IF) DAZL (Abcam, 34139, 1:400, IF)) OCT4 (BD, 611202, 1:200, IF) OCT4 (Abcam, 181557, 1:200, IF) SCP3 (Santa Cruz, SC-74569, 1:200, IF) LAMINA/C (Cell Signaling, 4777, 1:200, IF) LAMIN B1 (Santa Cruz, SC6216, 1:200, IF) LAMIN B1 (Santa Cruz, SC374015), 1:200, IF LBR (Abcam, Ab232731, 1:200, IF) Ki67 (Cell Signaling, 99129, 1:200, IF) ANTI-GFP (Abcam, Ab5450, 1:200, IF) AP2 gamma (Santa Cruz, SC-12762, 1:200, IF) ANTI-RFP (Abcam, 62341, 1:200, IF) Anti-human/mouse SSEA1 Alexa Fluor647 (ebioscience, 518813, 1:20, FACS) Goat anti mouse Alexafluor 488 (Thermo Fisher, A11029, 1:500, IF) Goat anti mouse Alexafluor 568 (Thermo Fisher, A21144, 1:500, IF) Goat anti mouse Alexafluor 647 (Thermo Fisher, A-21235, 1:500, IF) Goat anti rabbit Alexafluor 488 (Thermo Fisher, A11034, 1:500, IF) Goat anti Rabbit Alexafluor 568 (Thermo Fisher, A11036, 1:500, IF) Goat anti Rabbit Alexafluor 647 (Thermo Fisher, A21245, 1:500, IF) Donkey anti-Mouse IgG Secondary Antibody, HRP (Thermo Fisher, A16011, 1:10000, WB) Goat anti-Rabbit IgG Secondary Antibody, HRP (Thermo Fisher, A16096, 1:10000, WB)) Validation All antibodies were purchased from the commercial vendors and were validated by their vendors. .. AES0146 : Dnmt1-/-Dnmt3a-/-Dnmt3b-/- ES (clone19), (PMID: 16824199) J1 ES cells were purchased from ATCC(SCRC-1010).
Magnetic Resonance Imaging:Article Title:
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used AP2-alpha (Santa Cruz Biotechnology; sc-12726; clone 3B5; 1:200); AP2-gamma (R&D Systems; AF5059; 1:500); AP2-gamma (Santa Cruz Biotechnology, sc-12762; clone 6E4/4; 1:200); Brachyury (R&D Systems; AF2085; 1:500); CDX2 (BioGenex; MU392-UC; clone 4 nature portfolio | reporting sum m ary M arch 2021 CDX2-88; 1:200); CER1 (R&D Systems; AF1075; 1:500); Cytokeratin 7 (Aligent; M7018; clone OV-TL 12/30; 1:100); E-Cadherin (BD Biosciences; 610182; clone 36; 1:200); EOMES (Abcam; ab23345; 1:200); FOXA2 (R&D Systems; AF2400; 1:200); GATA2 (Novus Biologics; NBMP1-82581; 1:200); GATA3 (Abcam; ab199428; clone EPR16651; 1:500); GATA4 (Santa Cruz Biotechnology; sc-25310; clone G-4; 1:200); GATA4 (ThermoFisher Scientific; 14-9980-82; clone eBioEvan; 1:500); GATA6 (R&D Systems; AF1700; 1:500); GATA6 (Cell Signaling Technology; 5851; clone D61E4; 1:2000); GFP (Abcam; ab13970; 1:1000); GFP (Nacalai USA; GF090R; clone GF090R; 1:1000); HAND1 (DSHB; PCRP-HAND1-2A9; clone 2A9 1:200); HNF4-alpha (Abcam; ab201460; clone EPR16885-99; 1:2000); ISL1 (DSHB; PCRP-ISL1-1A9; clone 1A9; 1:100); Laminin (Sigma Aldrich; L9393; 1:200); N-Cadherin (Abcam; ab98952; clone 5D5; 1:200); NANOG (Cell Signaling Technology; 4903; clone D73G4; 1:200); OCT3/4 (Santa Cruz Biotechnology; sc-5279; clone C-10; 1:100); OTX2 (R&D Systems; AF1979; 1:1000); phospho-SMAD1/5 (Cell Signaling Technology; 9516S; clone 41D10; 1:200); Smad2/3 (Cell Signaling Technology; 8685S; clone D7G7; 1:200); SOX17 (R&D Systems; AF1924; 1:500); SOX2 (ThermoFisher Scientific; 14-9811-82; clone Btjce; 1:500); TBX20 (R&D Systems; MAB8124; clone 668710; 1:100); VTCN1 (Abcam; ab209242; clone EPR20236; 1:200). .. AlexaFluor-405 Donkey Anti-Mouse (ThermoFisher Scientific; A48257; 1:500); AlexaFluor-488 Donkey Anti-Rat (ThermoFisher Scientific; A-21208; 1:500); AlexFluor-488 Donkey Anti-Mouse (ThermoFisher Scientific; A-21202; 1:500); AlexaFluor-488 Donkey Anti-Goat (ThermoFisher Scientific; A-11055; 1:500); AlexaFluor-568 Donkey Anti-Rabbit (ThermoFisher Scientific; A10042; 1:500); AlexaFluor-568 Donkey Anti-Rat (ThermoFisher Scientific; A78946; 1:500); AlexaFluor-568 Donkey Anti-Mouse (ThermoFisher Scientific; A10037; 1:500); AlexaFluor-647 Donkey Anti-Rat (ThermoFisher Scientific; A78947; 1:500); AlexaFluor-647 Donkey AntiGoat (ThermoFisher Scientific; A-21447; 1:500); AlexaFluor-647 Donkey Anti-Rabbit (Thermofisher Scientific; A-31573; 1:500); AlexaFluor-647 Donkey Anti-Mouse (ThermoFisher Scientific; A32787; 1:500).
Article Title: Elucidating causative gene variants in hereditary Parkinson's disease in the Global Parkinson's Genetics Program (GP2).
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used AP2-alpha (Santa Cruz Biotechnology; sc-12726; clone 3B5; 1:200); AP2-gamma (R&D Systems; AF5059; 1:500); AP2-gamma (Santa Cruz Biotechnology, sc-12762; clone 6E4/4; 1:200); Brachyury (R&D Systems; AF2085; 1:500); CDX2 (BioGenex; MU392-UC; clone 4 nature portfolio | reporting sum m ary M arch 2021 CDX2-88; 1:200); CER1 (R&D Systems; AF1075; 1:500); Cytokeratin 7 (Aligent; M7018; clone OV-TL 12/30; 1:100); E-Cadherin (BD Biosciences; 610182; clone 36; 1:200); EOMES (Abcam; ab23345; 1:200); FOXA2 (R&D Systems; AF2400; 1:200); GATA2 (Novus Biologics; NBMP1-82581; 1:200); GATA3 (Abcam; ab199428; clone EPR16651; 1:500); GATA4 (Santa Cruz Biotechnology; sc-25310; clone G-4; 1:200); GATA4 (ThermoFisher Scientific; 14-9980-82; clone eBioEvan; 1:500); GATA6 (R&D Systems; AF1700; 1:500); GATA6 (Cell Signaling Technology; 5851; clone D61E4; 1:2000); GFP (Abcam; ab13970; 1:1000); GFP (Nacalai USA; GF090R; clone GF090R; 1:1000); HAND1 (DSHB; PCRP-HAND1-2A9; clone 2A9 1:200); HNF4-alpha (Abcam; ab201460; clone EPR16885-99; 1:2000); ISL1 (DSHB; PCRP-ISL1-1A9; clone 1A9; 1:100); Laminin (Sigma Aldrich; L9393; 1:200); N-Cadherin (Abcam; ab98952; clone 5D5; 1:200); NANOG (Cell Signaling Technology; 4903; clone D73G4; 1:200); OCT3/4 (Santa Cruz Biotechnology; sc-5279; clone C-10; 1:100); OTX2 (R&D Systems; AF1979; 1:1000); phospho-SMAD1/5 (Cell Signaling Technology; 9516S; clone 41D10; 1:200); Smad2/3 (Cell Signaling Technology; 8685S; clone D7G7; 1:200); SOX17 (R&D Systems; AF1924; 1:500); SOX2 (ThermoFisher Scientific; 14-9811-82; clone Btjce; 1:500); TBX20 (R&D Systems; MAB8124; clone 668710; 1:100); VTCN1 (Abcam; ab209242; clone EPR20236; 1:200). .. AlexaFluor-405 Donkey Anti-Mouse (ThermoFisher Scientific; A48257; 1:500); AlexaFluor-488 Donkey Anti-Rat (ThermoFisher Scientific; A-21208; 1:500); AlexFluor-488 Donkey Anti-Mouse (ThermoFisher Scientific; A-21202; 1:500); AlexaFluor-488 Donkey Anti-Goat (ThermoFisher Scientific; A-11055; 1:500); AlexaFluor-568 Donkey Anti-Rabbit (ThermoFisher Scientific; A10042; 1:500); AlexaFluor-568 Donkey Anti-Rat (ThermoFisher Scientific; A78946; 1:500); AlexaFluor-568 Donkey Anti-Mouse (ThermoFisher Scientific; A10037; 1:500); AlexaFluor-647 Donkey Anti-Rat (ThermoFisher Scientific; A78947; 1:500); AlexaFluor-647 Donkey AntiGoat (ThermoFisher Scientific; A-21447; 1:500); AlexaFluor-647 Donkey Anti-Rabbit (Thermofisher Scientific; A-31573; 1:500); AlexaFluor-647 Donkey Anti-Mouse (ThermoFisher Scientific; A32787; 1:500).
Article Title: Global reorganization of genome architecture at the transition to gametogenesis.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used H3K9me3 (Diagenode, C15410056, 1:400,IF) H3K9me3 (Diagenode, C15410193, 1:400,IF) H4K20me3 (Abcam, ab78517, 1:200, IF) H3K9me2 (Diagenode, C15410060, 1:200, IF) Pan-H3 (Abcam, Ab1791, 1:10000, WB) CENPA ((IF preferred), Cell signaling 2048,1:200, IF) CENPA (Cell Signaling, 2186T, for Human, 1:200, IF) CYCLIN B1 (Cell Signaling, 4138s, 1:200, IF) RAD21 (Abcam, 154769, 1:200, IF) CTCF (Cell Signaling, 2899, 1:200, IF) ATRX (Santa Cruz, SC15408, 1:200, IF) HP1alpha (Cell Signaling, 2616S, 1:200, IF) PCNA (Cell Signaling, 2586, 1:200, IF) MVH (Abcam, AB13840, 1:400, IF) MVH (Abcam, AB27591, 1:400, IF) DAZL (Abcam, 34139, 1:400, IF)) OCT4 (BD, 611202, 1:200, IF) OCT4 (Abcam, 181557, 1:200, IF) SCP3 (Santa Cruz, SC-74569, 1:200, IF) LAMINA/C (Cell Signaling, 4777, 1:200, IF) LAMIN B1 (Santa Cruz, SC6216, 1:200, IF) LAMIN B1 (Santa Cruz, SC374015), 1:200, IF LBR (Abcam, Ab232731, 1:200, IF) Ki67 (Cell Signaling, 99129, 1:200, IF) ANTI-GFP (Abcam, Ab5450, 1:200, IF) AP2 gamma (Santa Cruz, SC-12762, 1:200, IF) ANTI-RFP (Abcam, 62341, 1:200, IF) Anti-human/mouse SSEA1 Alexa Fluor647 (ebioscience, 518813, 1:20, FACS) Goat anti mouse Alexafluor 488 (Thermo Fisher, A11029, 1:500, IF) Goat anti mouse Alexafluor 568 (Thermo Fisher, A21144, 1:500, IF) Goat anti mouse Alexafluor 647 (Thermo Fisher, A-21235, 1:500, IF) Goat anti rabbit Alexafluor 488 (Thermo Fisher, A11034, 1:500, IF) Goat anti Rabbit Alexafluor 568 (Thermo Fisher, A11036, 1:500, IF) Goat anti Rabbit Alexafluor 647 (Thermo Fisher, A21245, 1:500, IF) Donkey anti-Mouse IgG Secondary Antibody, HRP (Thermo Fisher, A16011, 1:10000, WB) Goat anti-Rabbit IgG Secondary Antibody, HRP (Thermo Fisher, A16096, 1:10000, WB)) Validation All antibodies were purchased from the commercial vendors and were validated by their vendors. .. AES0146 : Dnmt1-/-Dnmt3a-/-Dnmt3b-/- ES (clone19), (PMID: 16824199) J1 ES cells were purchased from ATCC(SCRC-1010).
Western Blot:Article Title: Global reorganization of genome architecture at the transition to gametogenesis.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used H3K9me3 (Diagenode, C15410056, 1:400,IF) H3K9me3 (Diagenode, C15410193, 1:400,IF) H4K20me3 (Abcam, ab78517, 1:200, IF) H3K9me2 (Diagenode, C15410060, 1:200, IF) Pan-H3 (Abcam, Ab1791, 1:10000, WB) CENPA ((IF preferred), Cell signaling 2048,1:200, IF) CENPA (Cell Signaling, 2186T, for Human, 1:200, IF) CYCLIN B1 (Cell Signaling, 4138s, 1:200, IF) RAD21 (Abcam, 154769, 1:200, IF) CTCF (Cell Signaling, 2899, 1:200, IF) ATRX (Santa Cruz, SC15408, 1:200, IF) HP1alpha (Cell Signaling, 2616S, 1:200, IF) PCNA (Cell Signaling, 2586, 1:200, IF) MVH (Abcam, AB13840, 1:400, IF) MVH (Abcam, AB27591, 1:400, IF) DAZL (Abcam, 34139, 1:400, IF)) OCT4 (BD, 611202, 1:200, IF) OCT4 (Abcam, 181557, 1:200, IF) SCP3 (Santa Cruz, SC-74569, 1:200, IF) LAMINA/C (Cell Signaling, 4777, 1:200, IF) LAMIN B1 (Santa Cruz, SC6216, 1:200, IF) LAMIN B1 (Santa Cruz, SC374015), 1:200, IF LBR (Abcam, Ab232731, 1:200, IF) Ki67 (Cell Signaling, 99129, 1:200, IF) ANTI-GFP (Abcam, Ab5450, 1:200, IF) AP2 gamma (Santa Cruz, SC-12762, 1:200, IF) ANTI-RFP (Abcam, 62341, 1:200, IF) Anti-human/mouse SSEA1 Alexa Fluor647 (ebioscience, 518813, 1:20, FACS) Goat anti mouse Alexafluor 488 (Thermo Fisher, A11029, 1:500, IF) Goat anti mouse Alexafluor 568 (Thermo Fisher, A21144, 1:500, IF) Goat anti mouse Alexafluor 647 (Thermo Fisher, A-21235, 1:500, IF) Goat anti rabbit Alexafluor 488 (Thermo Fisher, A11034, 1:500, IF) Goat anti Rabbit Alexafluor 568 (Thermo Fisher, A11036, 1:500, IF) Goat anti Rabbit Alexafluor 647 (Thermo Fisher, A21245, 1:500, IF) Donkey anti-Mouse IgG Secondary Antibody, HRP (Thermo Fisher, A16011, 1:10000, WB) Goat anti-Rabbit IgG Secondary Antibody, HRP (Thermo Fisher, A16096, 1:10000, WB)) Validation All antibodies were purchased from the commercial vendors and were validated by their vendors. .. AES0146 : Dnmt1-/-Dnmt3a-/-Dnmt3b-/- ES (clone19), (PMID: 16824199) J1 ES cells were purchased from ATCC(SCRC-1010).
FACS:Article Title: Global reorganization of genome architecture at the transition to gametogenesis.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used H3K9me3 (Diagenode, C15410056, 1:400,IF) H3K9me3 (Diagenode, C15410193, 1:400,IF) H4K20me3 (Abcam, ab78517, 1:200, IF) H3K9me2 (Diagenode, C15410060, 1:200, IF) Pan-H3 (Abcam, Ab1791, 1:10000, WB) CENPA ((IF preferred), Cell signaling 2048,1:200, IF) CENPA (Cell Signaling, 2186T, for Human, 1:200, IF) CYCLIN B1 (Cell Signaling, 4138s, 1:200, IF) RAD21 (Abcam, 154769, 1:200, IF) CTCF (Cell Signaling, 2899, 1:200, IF) ATRX (Santa Cruz, SC15408, 1:200, IF) HP1alpha (Cell Signaling, 2616S, 1:200, IF) PCNA (Cell Signaling, 2586, 1:200, IF) MVH (Abcam, AB13840, 1:400, IF) MVH (Abcam, AB27591, 1:400, IF) DAZL (Abcam, 34139, 1:400, IF)) OCT4 (BD, 611202, 1:200, IF) OCT4 (Abcam, 181557, 1:200, IF) SCP3 (Santa Cruz, SC-74569, 1:200, IF) LAMINA/C (Cell Signaling, 4777, 1:200, IF) LAMIN B1 (Santa Cruz, SC6216, 1:200, IF) LAMIN B1 (Santa Cruz, SC374015), 1:200, IF LBR (Abcam, Ab232731, 1:200, IF) Ki67 (Cell Signaling, 99129, 1:200, IF) ANTI-GFP (Abcam, Ab5450, 1:200, IF) AP2 gamma (Santa Cruz, SC-12762, 1:200, IF) ANTI-RFP (Abcam, 62341, 1:200, IF) Anti-human/mouse SSEA1 Alexa Fluor647 (ebioscience, 518813, 1:20, FACS) Goat anti mouse Alexafluor 488 (Thermo Fisher, A11029, 1:500, IF) Goat anti mouse Alexafluor 568 (Thermo Fisher, A21144, 1:500, IF) Goat anti mouse Alexafluor 647 (Thermo Fisher, A-21235, 1:500, IF) Goat anti rabbit Alexafluor 488 (Thermo Fisher, A11034, 1:500, IF) Goat anti Rabbit Alexafluor 568 (Thermo Fisher, A11036, 1:500, IF) Goat anti Rabbit Alexafluor 647 (Thermo Fisher, A21245, 1:500, IF) Donkey anti-Mouse IgG Secondary Antibody, HRP (Thermo Fisher, A16011, 1:10000, WB) Goat anti-Rabbit IgG Secondary Antibody, HRP (Thermo Fisher, A16096, 1:10000, WB)) Validation All antibodies were purchased from the commercial vendors and were validated by their vendors. .. AES0146 : Dnmt1-/-Dnmt3a-/-Dnmt3b-/- ES (clone19), (PMID: 16824199) J1 ES cells were purchased from ATCC(SCRC-1010).
Biomarker Discovery:Article Title: Global reorganization of genome architecture at the transition to gametogenesis.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used H3K9me3 (Diagenode, C15410056, 1:400,IF) H3K9me3 (Diagenode, C15410193, 1:400,IF) H4K20me3 (Abcam, ab78517, 1:200, IF) H3K9me2 (Diagenode, C15410060, 1:200, IF) Pan-H3 (Abcam, Ab1791, 1:10000, WB) CENPA ((IF preferred), Cell signaling 2048,1:200, IF) CENPA (Cell Signaling, 2186T, for Human, 1:200, IF) CYCLIN B1 (Cell Signaling, 4138s, 1:200, IF) RAD21 (Abcam, 154769, 1:200, IF) CTCF (Cell Signaling, 2899, 1:200, IF) ATRX (Santa Cruz, SC15408, 1:200, IF) HP1alpha (Cell Signaling, 2616S, 1:200, IF) PCNA (Cell Signaling, 2586, 1:200, IF) MVH (Abcam, AB13840, 1:400, IF) MVH (Abcam, AB27591, 1:400, IF) DAZL (Abcam, 34139, 1:400, IF)) OCT4 (BD, 611202, 1:200, IF) OCT4 (Abcam, 181557, 1:200, IF) SCP3 (Santa Cruz, SC-74569, 1:200, IF) LAMINA/C (Cell Signaling, 4777, 1:200, IF) LAMIN B1 (Santa Cruz, SC6216, 1:200, IF) LAMIN B1 (Santa Cruz, SC374015), 1:200, IF LBR (Abcam, Ab232731, 1:200, IF) Ki67 (Cell Signaling, 99129, 1:200, IF) ANTI-GFP (Abcam, Ab5450, 1:200, IF) AP2 gamma (Santa Cruz, SC-12762, 1:200, IF) ANTI-RFP (Abcam, 62341, 1:200, IF) Anti-human/mouse SSEA1 Alexa Fluor647 (ebioscience, 518813, 1:20, FACS) Goat anti mouse Alexafluor 488 (Thermo Fisher, A11029, 1:500, IF) Goat anti mouse Alexafluor 568 (Thermo Fisher, A21144, 1:500, IF) Goat anti mouse Alexafluor 647 (Thermo Fisher, A-21235, 1:500, IF) Goat anti rabbit Alexafluor 488 (Thermo Fisher, A11034, 1:500, IF) Goat anti Rabbit Alexafluor 568 (Thermo Fisher, A11036, 1:500, IF) Goat anti Rabbit Alexafluor 647 (Thermo Fisher, A21245, 1:500, IF) Donkey anti-Mouse IgG Secondary Antibody, HRP (Thermo Fisher, A16011, 1:10000, WB) Goat anti-Rabbit IgG Secondary Antibody, HRP (Thermo Fisher, A16096, 1:10000, WB)) Validation All antibodies were purchased from the commercial vendors and were validated by their vendors. .. AES0146 : Dnmt1-/-Dnmt3a-/-Dnmt3b-/- ES (clone19), (PMID: 16824199) J1 ES cells were purchased from ATCC(SCRC-1010).
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